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(A) UMAP plot showing unbiased clustering of CD8+ T cells based on the immune markers CD103, CD69, CD27, CCR7, CD127, <t>CCR5,</t> CD28, HLA-DR, CD38, 287 PD1, CD39, TIGIT. (B) UMAP plots of expression levels of selected immune markers in TB-PE-derived CD8+ T cells. (C) Proportions of CD8+ T cell clusters identified by UMAP analysis. (D) Heatmap of average expression of 12 variably expressed markers across clusters. (E) scRNA/TCR-seq analysis of TB-PE-derived CD8+ T cells from one PLWH/TB, showing TCR specificities for viral antigens. Cytokine and cytotoxic signatures from scRNAseq data are shown (top), with a heatmap of TNF, IFNG, GZMA, and GZMB expression (bottom). (F) Exhaustion signature from scRNAseq data (top), with a heatmap showing relative expression of exhaustion-associated genes TIGIT, LAG3, CTLA4, PDCD1, and HAVCR2 (bottom).
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(A) UMAP plot showing unbiased clustering of CD8+ T cells based on the immune markers CD103, CD69, CD27, CCR7, CD127, <t>CCR5,</t> CD28, HLA-DR, CD38, 287 PD1, CD39, TIGIT. (B) UMAP plots of expression levels of selected immune markers in TB-PE-derived CD8+ T cells. (C) Proportions of CD8+ T cell clusters identified by UMAP analysis. (D) Heatmap of average expression of 12 variably expressed markers across clusters. (E) scRNA/TCR-seq analysis of TB-PE-derived CD8+ T cells from one PLWH/TB, showing TCR specificities for viral antigens. Cytokine and cytotoxic signatures from scRNAseq data are shown (top), with a heatmap of TNF, IFNG, GZMA, and GZMB expression (bottom). (F) Exhaustion signature from scRNAseq data (top), with a heatmap showing relative expression of exhaustion-associated genes TIGIT, LAG3, CTLA4, PDCD1, and HAVCR2 (bottom).
Anti Ccr5, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) UMAP plot showing unbiased clustering of CD8+ T cells based on the immune markers CD103, CD69, CD27, CCR7, CD127, <t>CCR5,</t> CD28, HLA-DR, CD38, 287 PD1, CD39, TIGIT. (B) UMAP plots of expression levels of selected immune markers in TB-PE-derived CD8+ T cells. (C) Proportions of CD8+ T cell clusters identified by UMAP analysis. (D) Heatmap of average expression of 12 variably expressed markers across clusters. (E) scRNA/TCR-seq analysis of TB-PE-derived CD8+ T cells from one PLWH/TB, showing TCR specificities for viral antigens. Cytokine and cytotoxic signatures from scRNAseq data are shown (top), with a heatmap of TNF, IFNG, GZMA, and GZMB expression (bottom). (F) Exhaustion signature from scRNAseq data (top), with a heatmap showing relative expression of exhaustion-associated genes TIGIT, LAG3, CTLA4, PDCD1, and HAVCR2 (bottom).
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(A) UMAP plot showing unbiased clustering of CD8+ T cells based on the immune markers CD103, CD69, CD27, CCR7, CD127, <t>CCR5,</t> CD28, HLA-DR, CD38, 287 PD1, CD39, TIGIT. (B) UMAP plots of expression levels of selected immune markers in TB-PE-derived CD8+ T cells. (C) Proportions of CD8+ T cell clusters identified by UMAP analysis. (D) Heatmap of average expression of 12 variably expressed markers across clusters. (E) scRNA/TCR-seq analysis of TB-PE-derived CD8+ T cells from one PLWH/TB, showing TCR specificities for viral antigens. Cytokine and cytotoxic signatures from scRNAseq data are shown (top), with a heatmap of TNF, IFNG, GZMA, and GZMB expression (bottom). (F) Exhaustion signature from scRNAseq data (top), with a heatmap showing relative expression of exhaustion-associated genes TIGIT, LAG3, CTLA4, PDCD1, and HAVCR2 (bottom).
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(A) UMAP plot showing unbiased clustering of CD8+ T cells based on the immune markers CD103, CD69, CD27, CCR7, CD127, <t>CCR5,</t> CD28, HLA-DR, CD38, 287 PD1, CD39, TIGIT. (B) UMAP plots of expression levels of selected immune markers in TB-PE-derived CD8+ T cells. (C) Proportions of CD8+ T cell clusters identified by UMAP analysis. (D) Heatmap of average expression of 12 variably expressed markers across clusters. (E) scRNA/TCR-seq analysis of TB-PE-derived CD8+ T cells from one PLWH/TB, showing TCR specificities for viral antigens. Cytokine and cytotoxic signatures from scRNAseq data are shown (top), with a heatmap of TNF, IFNG, GZMA, and GZMB expression (bottom). (F) Exhaustion signature from scRNAseq data (top), with a heatmap showing relative expression of exhaustion-associated genes TIGIT, LAG3, CTLA4, PDCD1, and HAVCR2 (bottom).
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(A) UMAP plot showing unbiased clustering of CD8+ T cells based on the immune markers CD103, CD69, CD27, CCR7, CD127, <t>CCR5,</t> CD28, HLA-DR, CD38, 287 PD1, CD39, TIGIT. (B) UMAP plots of expression levels of selected immune markers in TB-PE-derived CD8+ T cells. (C) Proportions of CD8+ T cell clusters identified by UMAP analysis. (D) Heatmap of average expression of 12 variably expressed markers across clusters. (E) scRNA/TCR-seq analysis of TB-PE-derived CD8+ T cells from one PLWH/TB, showing TCR specificities for viral antigens. Cytokine and cytotoxic signatures from scRNAseq data are shown (top), with a heatmap of TNF, IFNG, GZMA, and GZMB expression (bottom). (F) Exhaustion signature from scRNAseq data (top), with a heatmap showing relative expression of exhaustion-associated genes TIGIT, LAG3, CTLA4, PDCD1, and HAVCR2 (bottom).
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(A) UMAP plot showing unbiased clustering of CD8+ T cells based on the immune markers CD103, CD69, CD27, CCR7, CD127, <t>CCR5,</t> CD28, HLA-DR, CD38, 287 PD1, CD39, TIGIT. (B) UMAP plots of expression levels of selected immune markers in TB-PE-derived CD8+ T cells. (C) Proportions of CD8+ T cell clusters identified by UMAP analysis. (D) Heatmap of average expression of 12 variably expressed markers across clusters. (E) scRNA/TCR-seq analysis of TB-PE-derived CD8+ T cells from one PLWH/TB, showing TCR specificities for viral antigens. Cytokine and cytotoxic signatures from scRNAseq data are shown (top), with a heatmap of TNF, IFNG, GZMA, and GZMB expression (bottom). (F) Exhaustion signature from scRNAseq data (top), with a heatmap showing relative expression of exhaustion-associated genes TIGIT, LAG3, CTLA4, PDCD1, and HAVCR2 (bottom).
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sm cd21 bl13 fluidigm 3152010b 153 eu ccr5 j418f1 biolegend  (fluidigm)
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(A) UMAP plot showing unbiased clustering of CD8+ T cells based on the immune markers CD103, CD69, CD27, CCR7, CD127, <t>CCR5,</t> CD28, HLA-DR, CD38, 287 PD1, CD39, TIGIT. (B) UMAP plots of expression levels of selected immune markers in TB-PE-derived CD8+ T cells. (C) Proportions of CD8+ T cell clusters identified by UMAP analysis. (D) Heatmap of average expression of 12 variably expressed markers across clusters. (E) scRNA/TCR-seq analysis of TB-PE-derived CD8+ T cells from one PLWH/TB, showing TCR specificities for viral antigens. Cytokine and cytotoxic signatures from scRNAseq data are shown (top), with a heatmap of TNF, IFNG, GZMA, and GZMB expression (bottom). (F) Exhaustion signature from scRNAseq data (top), with a heatmap showing relative expression of exhaustion-associated genes TIGIT, LAG3, CTLA4, PDCD1, and HAVCR2 (bottom).
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Image Search Results


(A) UMAP plot showing unbiased clustering of CD8+ T cells based on the immune markers CD103, CD69, CD27, CCR7, CD127, CCR5, CD28, HLA-DR, CD38, 287 PD1, CD39, TIGIT. (B) UMAP plots of expression levels of selected immune markers in TB-PE-derived CD8+ T cells. (C) Proportions of CD8+ T cell clusters identified by UMAP analysis. (D) Heatmap of average expression of 12 variably expressed markers across clusters. (E) scRNA/TCR-seq analysis of TB-PE-derived CD8+ T cells from one PLWH/TB, showing TCR specificities for viral antigens. Cytokine and cytotoxic signatures from scRNAseq data are shown (top), with a heatmap of TNF, IFNG, GZMA, and GZMB expression (bottom). (F) Exhaustion signature from scRNAseq data (top), with a heatmap showing relative expression of exhaustion-associated genes TIGIT, LAG3, CTLA4, PDCD1, and HAVCR2 (bottom).

Journal: bioRxiv

Article Title: The tuberculosis-associated microenvironment promotes HIV-1 persistence by impairing CD8+ T cell-mediated viral control

doi: 10.1101/2025.10.14.682453

Figure Lengend Snippet: (A) UMAP plot showing unbiased clustering of CD8+ T cells based on the immune markers CD103, CD69, CD27, CCR7, CD127, CCR5, CD28, HLA-DR, CD38, 287 PD1, CD39, TIGIT. (B) UMAP plots of expression levels of selected immune markers in TB-PE-derived CD8+ T cells. (C) Proportions of CD8+ T cell clusters identified by UMAP analysis. (D) Heatmap of average expression of 12 variably expressed markers across clusters. (E) scRNA/TCR-seq analysis of TB-PE-derived CD8+ T cells from one PLWH/TB, showing TCR specificities for viral antigens. Cytokine and cytotoxic signatures from scRNAseq data are shown (top), with a heatmap of TNF, IFNG, GZMA, and GZMB expression (bottom). (F) Exhaustion signature from scRNAseq data (top), with a heatmap showing relative expression of exhaustion-associated genes TIGIT, LAG3, CTLA4, PDCD1, and HAVCR2 (bottom).

Article Snippet: To characterize the cellular composition of TB-PE the following antibodies were included in a high-parameter flow cytometry panel: CD28-BUV395 (CD28.8, BD Biosciences), CD8-BUV496 (SK1, BD Biosciences), HLADQ-BUV563 (TU169, BD Biosciences), CD161-BUV615 (HP-3G10, BD Biosciences), CD154-BUV737 (TRAP1, BD Biosciences), CD4-BUV805 (OKT4, BD Biosciences), CCR7-BV421 ( -LI-A, BD Biosciences), CD39-BV480 (TU66, BD Biosciences), HLADR-BV605 (G46-6, BD Biosciences), PD1-BV711 (EH12.1, BD Biosciences), CD27-BV786 (LI27, BD Biosciences), CCR5-VioB515 (REA245, Miltenyi Biotec), CD3-NovaFluorB610 (UCHT1, Thermo Fisher Scientific), CD69-BB700 (FN50, BD Biosciences), TIGIT-PE (A15153G, BioLegend), CD103-PEVio615 (REA803, Miltenyi Biotec), CD127-PECY7 (R34.34, Thermo Fisher Scientific), and CD38-APCFire810 (HIT2, BioLegend).

Techniques: Expressing, Derivative Assay